Bioinformatics at the tip of your fingers
A free hub of molecular biology tools for DNA, RNA and protein analysis — clean, translate, align, calculate and search, all in your browser.
128,103+Registered biological researchers
Hosted Tools
DNA/RNA
19 toolsCleaner
Clean your sequences from unwanted text marks and spaces, useful after retrieving sequences from online databases.
Translator
Translate your DNA sequences from 1 to 6 phases with formatted outputs and multiple genetic codes.
NetPlasmid
Draw colored plasmid maps in a few minutes straight from your browser.
ORF Viewer
Find ORF regions on your DNA sequence with graphical output on the 6 phases (direct and reverse).
Batch Codon Usage
Find codon usage and %GC from one or more sequences in batch.
Cutter
Quickly get the list of all enzyme restriction sites on your sequence.
Complementor
Get the complementary (reversed or not) of your DNA sequence.
EnzFinder
Search for commercial data about any enzyme: availability, cut sequence, references.
OligoCalc
Calculate your primer parameters: length, %GC, melting temperature (Tm), molecular weight.
Primer Designer
Design PCR primers around your template, or around a target region, and get ranked pairs with their Tm, GC and product size. Inspired by Primer3, with simplicity in mind.
Primeranneal
Search and visualize where your primers will hybridize on your DNA template, direct and reversed at once.
Virtual Gel
Simulate an agarose gel: digest your sequence with restriction enzymes and read the band pattern.
Dot Plot
Compare two sequences, or a sequence with itself, and reveal repeats and inverted repeats as diagonals.
Align
Align two or more DNA or protein sequences from FASTA: pairwise (global or local) or progressive multiple alignment with ClustalW-style options.
Reverse Translate
Back-translate a protein into a DNA coding sequence using the preferred codons of your host.
Sequence Stats
Base composition, GC content, GC skew, melting temperature and molecular weight of a sequence.
Repeat Finder
Find tandem repeats, microsatellites and CpG islands in your DNA sequence.
Bench Calculators
Length to mass, amount conversions, OD260 readings and dilution planning at the bench.
RNA Secondary Structure
Fold an RNA sequence into its minimum free energy secondary structure and draw it as a stem-and-loop diagram or a circular map.
Protein
11 toolsCleaner
Clean your sequences from unwanted text marks and spaces, useful after retrieving sequences from online databases.
PatSearch
Search PROSITE patterns on your protein sequence.
Plot Analysis
Generate hydropathy and antigenic plots (Kyte-Doolittle and Hopp-Woods).
Seqpainter
Color your amino-acid sequence according to their properties (default by hydrophobicity).
ProtCalc
Determine statistical, theoretical MW, pI and extinction data from your protein sequence.
Align
Align two or more DNA or protein sequences from FASTA: pairwise (global or local) or progressive multiple alignment with ClustalW-style options.
Reverse Translate
Back-translate a protein into a DNA coding sequence using the preferred codons of your host.
Bench Calculators
Length to mass, amount conversions, OD260 readings and dilution planning at the bench.
Peptide Cutter
Digest a protein in silico with trypsin, chymotrypsin or other proteases and read the peptide masses.
Helical Wheel
Project a peptide onto a helix wheel to see its hydrophobic face and hydrophobic moment.
Charge Curve
Net charge of a protein across the pH scale, with its isoelectric point marked.
File Processing
4 toolsArrays
Three tools in one: average and sort array values by reference, gather several arrays on one line per reference, and label an ordered list from your own database.
Axion MEA
Transpose Axion MEA raw files (Maestro 12, 48, 96 wells) into a single file easy to analyze and compare.
Format Converter
Convert sequences between FASTA, GenBank, EMBL and plain text while keeping annotations.
FASTQ Inspector
Read count, length distribution and per-base quality of a FASTQ file, with light trimming.