JustBio NetPlasmid JustBio NetPlasmid Import a DNA sequence to automatically generate its plasmid map Plasmid Sequence: Paste here the full plasmid sequence. Position 0 of the plasmid will be set at the start of this sequence. This import process, will detect ORFs with orientation, commercial restriction enzymes positions and common promoters. Copy-paste DNA sequence here: Restriction Enzymes Selection All Available Enzymes: Restriction Enzymes to Use: AarI (CACCTGCNNNN^)AarI (^NNNNNNNNGCAGGTG)AasI (GACNNNN^NNGTC)AatI (AGG^CCT)AatII (GACGT^C)AauI (T^GTACA)AccI (GT^MKAC)AccII (CG^CG)AccIII (T^CCGGA)Acc16I (TGC^GCA)Acc36I (ACCTGCNNNN^)Acc36I (^NNNNNNNNGCAGGT)Acc65I (G^GTACC)Acc113I (AGT^ACT)AccB1I (G^GYRCC)AccB7I (CCANNNN^NTGG)AccBSI (CCG^CTC)AccBSI (GAG^CGG)AciI (C^CGC)AciI (G^CGG)AclI (AA^CGTT)AclWI (GGATCNNNN^)AclWI (^NNNNNGATCC)AcsI (R^AATTY)AcuI (CTGAAGNNNNNNNNNNNNNNNN^)AcuI (^NNNNNNNNNNNNNNCTTCAG)AcvI (CAC^GTG)AcyI (GR^CGYC)AdeI (CACNNN^GTG)AfaI (GT^AC)AfeI (AGC^GCT)AflI (G^GWCC)AflII (C^TTAAG)AflIII (A^CRYGT)AgeI (A^CCGGT)AhdI (GACNNN^NNGTC)AhlI (A^CTAGT)AleI (CACNN^NNGTG)AloI (GAACNNNNNNTCCNNNNNNNNNNNN^)AloI (^NNNNNNNGGANNNNNNGTTC)AloI (GGANNNNNNGTTCNNNNNNNNNNNN^)AloI (^NNNNNNNGAACNNNNNNTCC)AluI (AG^CT)AlwI (GGATCNNNN^)AlwI (^NNNNNGATCC)Alw21I (GWGCW^C)Alw26I (GTCTCN^)Alw26I (^NNNNNGAGAC)Alw44I (G^TGCAC)AlwNI (CAGNNN^CTG)Ama87I (C^YCGRG)AocI (CC^TNAGG)Aor51HI (AGC^GCT)ApaI (GGGCC^C)ApaLI (G^TGCAC)ApoI (R^AATTY)AscI (GG^CGCGCC)AseI (AT^TAAT)AsiAI (A^CCGGT)AsiSI (GCGAT^CGC)AsnI (AT^TAAT)AspI (GACN^NNGTC)Asp700I (GAANN^NNTTC)Asp718I (G^GTACC)AspEI (GACNNN^NNGTC)AspHI (GWGCW^C)AspLEI (GCG^C)AspS9I (G^GNCC)AsuII (TT^CGAA)AsuC2I (CC^SGG)AsuHPI (GGTGANNNNNNNN^)AsuHPI (^NNNNNNNTCACC)AsuNHI (G^CTAGC)AvaI (C^YCGRG)AvaII (G^GWCC)AviII (TGC^GCA)AvrII (C^CTAGG)AxyI (CC^TNAGG)BaeI (ACNNNNGTAYCNNNNNNNNNNNN^)BaeI (^NNNNNNNGRTACNNNNGT)BaeI (GRTACNNNNGTNNNNNNNNNNNNNNN^)BaeI (^NNNNNNNNNNACNNNNGTAYC)BalI (TGG^CCA)BamHI (G^GATCC)BanI (G^GYRCC)BanII (GRGCY^C)BanIII (AT^CGAT)BbeI (GGCGC^C)BbrPI (CAC^GTG)BbsI (GAAGACNN^)BbsI (^NNNNNNGTCTTC)BbuI (GCATG^C)BbvI (GCAGCNNNNNNNN^)BbvI (^NNNNNNNNNNNNGCTGC)Bbv12I (GWGCW^C)BbvCI (CC^TCAGC)BbvCI (GC^TGAGG)BceAI (ACGGCNNNNNNNNNNNN^)BceAI (^NNNNNNNNNNNNNNGCCGT)BcgI (CGANNNNNNTGCNNNNNNNNNNNN^)BcgI (^NNNNNNNNNNGCANNNNNNTCG)BcgI (GCANNNNNNTCGNNNNNNNNNNNN^)BcgI (^NNNNNNNNNNCGANNNNNNTGC)BciVI (GTATCCNNNNNN^)BciVI (^NNNNNGGATAC)BclI (T^GATCA)BcnI (CC^SGG)BcoI (C^YCGRG)BcuI (A^CTAGT)BfaI (C^TAG)BfiI (ACTGGGNNNNN^)BfiI (^NNNNCCCAGT)BfmI (C^TRYAG)BfrI (C^TTAAG)BfrBI (ATG^CAT)BfuI (GTATCCNNNNNN^)BfuI (^NNNNNGGATAC)BfuAI (ACCTGCNNNN^)BfuAI (^NNNNNNNNGCAGGT)BfuCI (^GATC)BglI (GCCNNNN^NGGC)BglII (A^GATCT)BlnI (C^CTAGG)BlpI (GC^TNAGC)Bme18I (G^GWCC)Bme1390I (CC^NGG)Bme1580I (GKGCM^C)BmgBI (CAC^GTC)BmgBI (GAC^GTG)BmrI (ACTGGGNNNNN^)BmrI (^NNNNCCCAGT)BmtI (GCTAG^C)BmyI (GDGCH^C)BoxI (GACNN^NNGTC)BpiI (GAAGACNN^)BpiI (^NNNNNNGTCTTC)BplI (GAGNNNNNCTCNNNNNNNNNNNNN^)BplI (^NNNNNNNNGAGNNNNNCTC)BplI (GAGNNNNNCTCNNNNNNNNNNNNN^)BplI (^NNNNNNNNGAGNNNNNCTC)BpmI (CTGGAGNNNNNNNNNNNNNNNN^)BpmI (^NNNNNNNNNNNNNNCTCCAG)Bpu10I (CC^TNAGC)Bpu10I (GC^TNAGG)Bpu14I (TT^CGAA)Bpu1102I (GC^TNAGC)BpuAI (GAAGACNN^)BpuAI (^NNNNNNGTCTTC)BpuEI (CTTGAGNNNNNNNNNNNNNNNN^)BpuEI (^NNNNNNNNNNNNNNCTCAAG)BsaI (GGTCTCN^)BsaI (^NNNNNGAGACC)Bsa29I (AT^CGAT)BsaAI (YAC^GTR)BsaBI (GATNN^NNATC)BsaHI (GR^CGYC)BsaJI (C^CNNGG)BsaMI (GAATGCN^)BsaMI (G^CATTC)BsaOI (CGRY^CG)BsaWI (W^CCGGW)BsaXI (ACNNNNNCTCCNNNNNNNNNN^)BsaXI (^NNNNNNNGGAGNNNNNGT)BsaXI (GGAGNNNNNGTNNNNNNNNNNNN^)BsaXI (^NNNNNNNNNACNNNNNCTCC)BscI (AT^CGAT)Bsc4I (CCNNNNN^NNGG)BscBI (GGN^NCC)BscCI (GAATGCN^)BscCI (G^CATTC)BscFI (^GATC)Bse1I (ACTGGN^)Bse1I (C^CAGT)Bse8I (GATNN^NNATC)Bse21I (CC^TNAGG)Bse118I (R^CCGGY)BseAI (T^CCGGA)BseBI (CC^WGG)BseCI (AT^CGAT)BseDI (C^CNNGG)Bse3DI (GCAATGNN^)Bse3DI (^CATTGC)BseGI (GGATGNN^)BseGI (^CATCC)BseJI (GATNN^NNATC)BseLI (CCNNNNN^NNGG)BseMI (GCAATGNN^)BseMI (^CATTGC)BseMII (CTCAGNNNNNNNNNN^)BseMII (^NNNNNNNNCTGAG)BseNI (ACTGGN^)BseNI (C^CAGT)BsePI (G^CGCGC)BseRI (GAGGAGNNNNNNNNNN^)BseRI (^NNNNNNNNCTCCTC)BseSI (GKGCM^C)BseXI (GCAGCNNNNNNNN^)BseXI (^NNNNNNNNNNNNGCTGC)BseX3I (C^GGCCG)BseYI (C^CCAGC)BseYI (G^CTGGG)BsgI (GTGCAGNNNNNNNNNNNNNNNN^)BsgI (^NNNNNNNNNNNNNNCTGCAC)BshI (GG^CC)Bsh1236I (CG^CG)Bsh1285I (CGRY^CG)BshFI (GG^CC)BshNI (G^GYRCC)BshTI (A^CCGGT)BsiBI (GATNN^NNATC)BsiCI (TT^CGAA)BsiEI (CGRY^CG)BsiHKAI (GWGCW^C)BsiHKCI (C^YCGRG)BsiLI (CC^WGG)BsiMI (T^CCGGA)BsiQI (T^GATCA)BsiSI (C^CGG)BsiWI (C^GTACG)BsiXI (AT^CGAT)BsiYI (CCNNNNN^NNGG)BsiZI (G^GNCC)BslI (CCNNNNN^NNGG)BsmI (GAATGCN^)BsmI (G^CATTC)BsmAI (GTCTCN^)BsmAI (^NNNNNGAGAC)BsmBI (CGTCTCN^)BsmBI (^NNNNNGAGACG)BsmFI (GGGACNNNNNNNNNN^)BsmFI (^NNNNNNNNNNNNNNGTCCC)Bso31I (GGTCTCN^)Bso31I (^NNNNNGAGACC)BsoBI (C^YCGRG)BsoMAI (GTCTCN^)BsoMAI (^NNNNNGAGAC)Bsp13I (T^CCGGA)Bsp19I (C^CATGG)Bsp68I (TCG^CGA)Bsp106I (AT^CGAT)Bsp119I (TT^CGAA)Bsp120I (G^GGCCC)Bsp143I (^GATC)Bsp143II (RGCGC^Y)Bsp1286I (GDGCH^C)Bsp1407I (T^GTACA)Bsp1720I (GC^TNAGC)BspA2I (C^CTAGG)BspCI (CGAT^CG)BspCNI (CTCAGNNNNNNNNN^)BspCNI (^NNNNNNNCTGAG)BspDI (AT^CGAT)BspEI (T^CCGGA)BspHI (T^CATGA)BspLI (GGN^NCC)BspLU11I (A^CATGT)BspMI (ACCTGCNNNN^)BspMI (^NNNNNNNNGCAGGT)BspPI (GGATCNNNN^)BspPI (^NNNNNGATCC)BspTI (C^TTAAG)BspT104I (TT^CGAA)BspT107I (G^GYRCC)BspTNI (GGTCTCN^)BspTNI (^NNNNNGAGACC)BspXI (AT^CGAT)BsrI (ACTGGN^)BsrI (C^CAGT)BsrBI (CCG^CTC)BsrBI (GAG^CGG)BsrBRI (GATNN^NNATC)BsrDI (GCAATGNN^)BsrDI (^CATTGC)BsrFI (R^CCGGY)BsrGI (T^GTACA)BsrSI (ACTGGN^)BsrSI (C^CAGT)BssAI (R^CCGGY)BssECI (C^CNNGG)BssHI (C^TCGAG)BssHII (G^CGCGC)BssKI (^CCNGG)BssNAI (GTA^TAC)BssSI (C^ACGAG)BssT1I (C^CWWGG)Bst6I (CTCTTCN^)Bst6I (^NNNNGAAGAG)Bst71I (GCAGCNNNNNNNN^)Bst71I (^NNNNNNNNNNNNGCTGC)Bst98I (C^TTAAG)Bst1107I (GTA^TAC)BstACI (GR^CGYC)BstAPI (GCANNNN^NTGC)BstBI (TT^CGAA)Bst2BI (C^ACGAG)Bst2BI (C^TCGTG)BstBAI (YAC^GTR)Bst4CI (ACN^GT)BstC8I (GCN^NGC)BstDEI (C^TNAG)BstDSI (C^CRYGG)BstEII (G^GTNACC)BstENI (CCTNN^NNNAGG)BstENII (^GATC)BstF5I (GGATGNN^)BstF5I (^CATCC)BstFNI (CG^CG)BstH2I (RGCGC^Y)BstHHI (GCG^C)BstHPI (GTT^AAC)BstKTI (GAT^C)BstMAI (CTGCA^G)BstMCI (CGRY^CG)BstMWI (GCNNNNN^NNGC)BstNI (CC^WGG)BstNSI (RCATG^Y)BstOI (CC^WGG)BstPI (G^GTNACC)BstPAI (GACNN^NNGTC)BstSCI (^CCNGG)BstSFI (C^TRYAG)BstSNI (TAC^GTA)BstUI (CG^CG)Bst2UI (CC^WGG)BstV1I (GCAGCNNNNNNNN^)BstV1I (^NNNNNNNNNNNNGCTGC)BstV2I (GAAGACNN^)BstV2I (^NNNNNNGTCTTC)BstXI (CCANNNNN^NTGG)BstX2I (R^GATCY)BstYI (R^GATCY)BstZI (C^GGCCG)BstZ17I (GTA^TAC)Bsu15I (AT^CGAT)Bsu36I (CC^TNAGG)BsuRI (GG^CC)BsuTUI (AT^CGAT)BtgI (C^CRYGG)BtrI (CAC^GTC)BtrI (GAC^GTG)BtsI (GCAGTGNN^)BtsI (^CACTGC)Cac8I (GCN^NGC)CaiI (CAGNNN^CTG)CciNI (GC^GGCCGC)CelII (GC^TNAGC)CfoI (GCG^C)CfrI (Y^GGCCR)Cfr9I (C^CCGGG)Cfr10I (R^CCGGY)Cfr13I (G^GNCC)Cfr42I (CCGC^GG)ClaI (AT^CGAT)CpoI (CG^GWCCG)CspI (CG^GWCCG)Csp6I (G^TAC)Csp45I (TT^CGAA)CspAI (A^CCGGT)CviJI (RG^CY)CviRI (TG^CA)CviTI (RG^CY)CvnI (CC^TNAGG)DdeI (C^TNAG)DpnI (GA^TC)DpnII (^GATC)DraI (TTT^AAA)DraII (RG^GNCCY)DraIII (CACNNN^GTG)DrdI (GACNNNN^NNGTC)DsaI (C^CRYGG)DseDI (GACNNNN^NNGTC)EaeI (Y^GGCCR)EagI (C^GGCCG)Eam1104I (CTCTTCN^)Eam1104I (^NNNNGAAGAG)Eam1105I (GACNNN^NNGTC)EarI (CTCTTCN^)EarI (^NNNNGAAGAG)EciI (GGCGGANNNNNNNNNNN^)EciI (^NNNNNNNNNTCCGCC)Ecl136II (GAG^CTC)EclHKI (GACNNN^NNGTC)EclXI (C^GGCCG)Eco24I (GRGCY^C)Eco31I (GGTCTCN^)Eco31I (^NNNNNGAGACC)Eco32I (GAT^ATC)Eco47I (G^GWCC)Eco47III (AGC^GCT)Eco52I (C^GGCCG)Eco57I (CTGAAGNNNNNNNNNNNNNNNN^)Eco57I (^NNNNNNNNNNNNNNCTTCAG)Eco72I (CAC^GTG)Eco81I (CC^TNAGG)Eco88I (C^YCGRG)Eco91I (G^GTNACC)Eco105I (TAC^GTA)Eco130I (C^CWWGG)Eco147I (AGG^CCT)EcoICRI (GAG^CTC)Eco57MI (CTGRAGNNNNNNNNNNNNNNNN^)Eco57MI (^NNNNNNNNNNNNNNCTYCAG)EcoNI (CCTNN^NNNAGG)EcoO65I (G^GTNACC)EcoO109I (RG^GNCCY)EcoRI (G^AATTC)EcoRII (^CCWGG)EcoRV (GAT^ATC)EcoT14I (C^CWWGG)EcoT22I (ATGCA^T)EcoT38I (GRGCY^C)EgeI (GGC^GCC)EheI (GGC^GCC)ErhI (C^CWWGG)Esp3I (CGTCTCN^)Esp3I (^NNNNNGAGACG)FalI (AAGNNNNNCTTNNNNNNNNNNNNN^)FalI (^NNNNNNNNAAGNNNNNCTT)FalI (AAGNNNNNCTTNNNNNNNNNNNNN^)FalI (^NNNNNNNNAAGNNNNNCTT)FatI (^CATG)FauI (CCCGCNNNN^)FauI (^NNNNNNGCGGG)FauNDI (CA^TATG)FbaI (T^GATCA)FblI (GT^MKAC)Fnu4HI (GC^NGC)FokI (GGATGNNNNNNNNN^)FokI (^NNNNNNNNNNNNNCATCC)FriOI (GRGCY^C)FseI (GGCCGG^CC)FspI (TGC^GCA)FspAI (RTGC^GCAY)Fsp4HI (GC^NGC)FunI (AGC^GCT)FunII (G^AATTC)GsuI (CTGGAGNNNNNNNNNNNNNNNN^)GsuI (^NNNNNNNNNNNNNNCTCCAG)HaeII (RGCGC^Y)HaeIII (GG^CC)HapII (C^CGG)HgaI (GACGCNNNNN^)HgaI (^NNNNNNNNNNGCGTC)HhaI (GCG^C)Hin1I (GR^CGYC)Hin4I (GAYNNNNNVTCNNNNNNNNNNNNN^)Hin4I (^NNNNNNNNGABNNNNNRTC)Hin4I (GABNNNNNRTCNNNNNNNNNNNNN^)Hin4I (^NNNNNNNNGAYNNNNNVTC)Hin6I (G^CGC)HinP1I (G^CGC)HincII (GTY^RAC)HindII (GTY^RAC)HindIII (A^AGCTT)HinfI (G^ANTC)HpaI (GTT^AAC)HpaII (C^CGG)HphI (GGTGANNNNNNNN^)HphI (^NNNNNNNTCACC)Hpy8I (GTN^NAC)Hpy99I (CGWCG^)Hpy188I (TCN^GA)Hpy188III (TC^NNGA)HpyCH4III (ACN^GT)HpyCH4IV (A^CGT)HpyCH4V (TG^CA)HpyF10VI (GCNNNNNN^NGC)Hsp92I (GR^CGYC)Hsp92II (CATG^)HspAI (G^CGC)ItaI (GC^NGC)KasI (G^GCGCC)KpnI (GGTAC^C)Kpn2I (T^CCGGA)KspI (CCGC^GG)Ksp22I (T^GATCA)Ksp632I (CTCTTCN^)Ksp632I (^NNNNGAAGAG)KspAI (GTT^AAC)Kzo9I (^GATC)LspI (TT^CGAA)LweI (GCATCNNNNN^)LweI (^NNNNNNNNNGATGC)MabI (A^CCWGGT)MaeI (C^TAG)MaeII (A^CGT)MaeIII (^GTNAC)MamI (GATNN^NNATC)MbiI (CCG^CTC)MbiI (GAG^CGG)MboI (^GATC)MboII (GAAGANNNNNNNN^)MboII (^NNNNNNNTCTTC)MfeI (C^AATTG)MflI (R^GATCY)MhlI (GDGCH^C)MlsI (TGG^CCA)MluI (A^CGCGT)MluNI (TGG^CCA)MlyI (GAGTCNNNNN^)MlyI (^NNNNNGACTC)Mly113I (GG^CGCC)MnlI (CCTCNNNNNNN^)MnlI (^NNNNNNGAGG)Mph1103I (ATGCA^T)MroI (T^CCGGA)MroNI (G^CCGGC)MroXI (GAANN^NNTTC)MscI (TGG^CCA)MseI (T^TAA)MslI (CAYNN^NNRTG)MspI (C^CGG)Msp20I (TGG^CCA)MspA1I (CMG^CKG)MspCI (C^TTAAG)MspR9I (CC^NGG)MssI (GTTT^AAAC)MunI (C^AATTG)MvaI (CC^WGG)Mva1269I (GAATGCN^)Mva1269I (G^CATTC)MvnI (CG^CG)MwoI (GCNNNNN^NNGC)NaeI (GCC^GGC)NarI (GG^CGCC)NciI (CC^SGG)NcoI (C^CATGG)NdeI (CA^TATG)NdeII (^GATC)NgoAIV (G^CCGGC)NgoMIV (G^CCGGC)NheI (G^CTAGC)NlaIII (CATG^)NlaIV (GGN^NCC)NmuCI (^GTSAC)NotI (GC^GGCCGC)NruI (TCG^CGA)NruGI (GACNNN^NNGTC)NsbI (TGC^GCA)NsiI (ATGCA^T)NspI (RCATG^Y)NspIII (C^YCGRG)NspV (TT^CGAA)OliI (CACNN^NNGTG)PacI (TTAAT^TAA)PaeI (GCATG^C)PaeR7I (C^TCGAG)PagI (T^CATGA)PalI (GG^CC)PauI (G^CGCGC)PceI (AGG^CCT)PciI (A^CATGT)PctI (GAATGCN^)PctI (G^CATTC)PdiI (GCC^GGC)PdmI (GAANN^NNTTC)Pfl23II (C^GTACG)PflBI (CCANNNN^NTGG)PflFI (GACN^NNGTC)PflMI (CCANNNN^NTGG)PinAI (A^CCGGT)PleI (GAGTCNNNN^)PleI (^NNNNNGACTC)Ple19I (CGAT^CG)PmaCI (CAC^GTG)PmeI (GTTT^AAAC)PmlI (CAC^GTG)PpiI (GAACNNNNNCTCNNNNNNNNNNNNN^)PpiI (^NNNNNNNNGAGNNNNNGTTC)PpiI (GAGNNNNNGTTCNNNNNNNNNNNN^)PpiI (^NNNNNNNGAACNNNNNCTC)PpsI (GAGTCNNNN^)PpsI (^NNNNNGACTC)Ppu10I (A^TGCAT)PpuMI (RG^GWCCY)PpuXI (RG^GWCCY)PshAI (GACNN^NNGTC)PshBI (AT^TAAT)PsiI (TTA^TAA)Psp5II (RG^GWCCY)Psp6I (^CCWGG)Psp1406I (AA^CGTT)PspAI (C^CCGGG)Psp124BI (GAGCT^C)PspEI (G^GTNACC)PspGI (^CCWGG)PspLI (C^GTACG)PspN4I (GGN^NCC)PspOMI (G^GGCCC)PspPI (G^GNCC)PspPPI (RG^GWCCY)PsrI (GAACNNNNNNTACNNNNNNNNNNNN^)PsrI (^NNNNNNNGTANNNNNNGTTC)PsrI (GTANNNNNNGTTCNNNNNNNNNNNN^)PsrI (^NNNNNNNGAACNNNNNNTAC)PstI (CTGCA^G)PsuI (R^GATCY)PsyI (GACN^NNGTC)PvuI (CGAT^CG)PvuII (CAG^CTG)RcaI (T^CATGA)RsaI (GT^AC)RsrII (CG^GWCCG)Rsr2I (CG^GWCCG)SacI (GAGCT^C)SacII (CCGC^GG)SalI (G^TCGAC)SanDI (GG^GWCCC)SapI (GCTCTTCN^)SapI (^NNNNGAAGAGC)SatI (GC^NGC)Sau96I (G^GNCC)Sau3AI (^GATC)SbfI (CCTGCA^GG)ScaI (AGT^ACT)SchI (GAGTCNNNNN^)SchI (^NNNNNGACTC)ScrFI (CC^NGG)SdaI (CCTGCA^GG)SduI (GDGCH^C)SexAI (A^CCWGGT)SfaNI (GCATCNNNNN^)SfaNI (^NNNNNNNNNGATGC)SfcI (C^TRYAG)SfiI (GGCCNNNN^NGGCC)SfoI (GGC^GCC)Sfr274I (C^TCGAG)Sfr303I (CCGC^GG)SfuI (TT^CGAA)SgfI (GCGAT^CGC)SgrAI (CR^CCGGYG)SgrBI (CCGC^GG)SinI (G^GWCC)SlaI (C^TCGAG)SmaI (CCC^GGG)SmiI (ATTT^AAAT)SmiMI (CAYNN^NNRTG)SmlI (C^TYRAG)SmuI (CCCGCNNNN^)SmuI (^NNNNNNGCGGG)SnaBI (TAC^GTA)SpaHI (GCATG^C)SpeI (A^CTAGT)SphI (GCATG^C)SrfI (GCCC^GGGC)Sse9I (^AATT)Sse8387I (CCTGCA^GG)SseBI (AGG^CCT)SspI (AAT^ATT)SspBI (T^GTACA)SstI (GAGCT^C)SstII (CCGC^GG)StuI (AGG^CCT)StyI (C^CWWGG)SunI (C^GTACG)SwaI (ATTT^AAAT)TaaI (ACN^GT)TaiI (ACGT^)TaqI (T^CGA)TaqII (GACCGANNNNNNNNNNN^)TaqII (^NNNNNNNNNTCGGTC)TaqII (CACCCANNNNNNNNNNN^)TaqII (^NNNNNNNNNTGGGTG)TasI (^AATT)TatI (W^GTACW)TauI (GCSG^C)TelI (GACN^NNGTC)TfiI (G^AWTC)ThaI (CG^CG)TliI (C^TCGAG)Tru1I (T^TAA)Tru9I (T^TAA)TscI (ACGT^)TseI (G^CWGC)Tsp45I (^GTSAC)Tsp509I (^AATT)TspEI (^AATT)TspGWI (ACGGANNNNNNNNNNN^)TspGWI (^NNNNNNNNNTCCGT)TspRI (CASTGNN^)Tth111I (GACN^NNGTC)TthHB8I (T^CGA)Van91I (CCANNNN^NTGG)Vha464I (C^TTAAG)VneI (G^TGCAC)VpaK11BI (G^GWCC)VspI (AT^TAAT)XagI (CCTNN^NNNAGG)XapI (R^AATTY)XbaI (T^CTAGA)XceI (RCATG^Y)XcmI (CCANNNNN^NNNNTGG)XhoI (C^TCGAG)XhoII (R^GATCY)XmaI (C^CCGGG)XmaIII (C^GGCCG)XmaCI (C^CCGGG)XmaJI (C^CTAGG)XmiI (GT^MKAC)XmnI (GAANN^NNTTC)XspI (C^TAG)ZhoI (AT^CGAT)ZraI (GAC^GTC)Zsp2I (ATGCA^T) ApaI (GGGCC^C)BamHI (G^GATCC)HindIII (A^AGCTT)NdeI (CA^TATG)PstI (CTGCA^G)SacII (CCGC^GG)XhoI (C^TCGAG) ORF cut-off Codon table nt (ORFs smaller than the cut-off will be ignored) Standard Genetic Code Vertebrate Mitochondrial Yeast Mitochondrial Invertebrate Mitochondrial Echinoderm Mitochondrial Alternative Yeast Nuclear